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SST5 (IHC-grade), Somatostatin Receptor 5 Antibody

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KO-Validated
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  • 7TM0359N-IC
  • 100 µl
  • Rabbit
The SST5 receptor antibody is directed against the distal end of the carboxyl-terminal tail of... more

The SST5 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human Somatostatin Receptor 5. In can be used to detect total SST5 receptors in Western blots independent of phosphorylation. The SST5 antibody can also be used to isolate and enrich SST5 receptors from cell and tissue lysates. It also detects SST5 in cultured cells and tissue sections by immunohistochemistry. The SST5 antibody has been validated using siRNA knockdown (KO-Validated).

  Alternative Names SST5, SSTR5, Somatostatin Receptor 5 IUPHAR... more

 

Alternative Names SST5, SSTR5, Somatostatin Receptor 5
IUPHAR Target ID  359
UniProt ID  P35346
Western Blot (WB)  1:1000
Immunocytochemistry (ICC) 1:200
Immunohistochemistry (IHC) 1:100
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic peptide with sequence QEATPPAHRAAANGLMQTSKL corresponds to amino acids 344-364 of human SST5
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Immunohistochemical identification of Somatostatin Receptor 5 in human pancreatic... more

Figure 1. Immunohistochemical identification of Somatostatin Receptor 5 in human pancreatic islets. Sections were dewaxed, microwaved in citric acid, and incubated with anti-SST5 (Somatostatin Receptor 5) antibody (7TM0359N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in AEC, and sections were counterstained with hematoxylin. Note, SST5 receptors were detected at the plasma membrane of nearly all cells in human pancreatic islets.

Figure 2. Immunohistochemical identification of Somatostatin Receptor 5 in human anterior pituitary. Sections were dewaxed, microwaved in citric acid, and incubated with anti-SST5 (Somatostatin Receptor 5) antibody (7TM0359N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, SST5 receptors were detected on a distinct population of cells in the anterior pituitary.

Figure 3. Immunohistochemical identification of Somatostatin Receptor 5 in human pheochromocytoma. Sections were dewaxed, microwaved in citric acid, and incubated with anti-SST5 (Somatostatin Receptor 5) antibody (7TM0359N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin. Note, SST5 receptors were uniformly detected at the plasma membrane of nearly all cells in pheochromocytoma.

Figure 4. Immunohistochemical identification of Somatostatin Receptor 5 in human growth-hormone-producing pituitary adenoma tissue. Sections were dewaxed, microwaved in citric acid, and incubated with anti-SST5 (Somatostatin Receptor 5) antibody (7TM0359N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, SST5 receptors were uniformly detected at the plasma membrane of nearly all tumor cells.

Figure 5. Immunocytochemical identification of Somatostatin Receptor 5 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Somatostatin Receptor 5 (SST5) were immunocytochemically stained with anti-SST5 (Somatostatin Receptor 5) antibody (7TM0359N-IC) at a dilution of 1:200. Note, SST5 receptors were confined to the plasma membrane in tranfected cells.

Figure 6. Western blot analysis of SST5 receptors in neuroendocrine tumors. Human tumor samples (T1, T2) from neuroendocrine tumors were homogenated and immunoblotted using anti-SST5 (Somatostatin-Receptor 5) antibody (7TM0359N-IC) at a dilution of 1:1000.

Figure 7. Validation of the Somatostatin Receptor 5 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Somatostatin Receptor 5 (SST5) were lysed and immunoblotted with the anti-SST5 antibody (7TM0359N-WB) at a dilution of 1:1000.

Schulz S, Pauli SU, Schulz S, Händel M, Dietzmann K, Firsching R, Höllt V. Immunohistochemical... more

Schulz S, Pauli SU, Schulz S, Händel M, Dietzmann K, Firsching R, Höllt V. Immunohistochemical determination of five somatostatin receptors in meningioma reveals frequent overexpression of somatostatin receptor subtype sst2A. Clin Cancer Res. 2000 May;6(5):1865-74. PubMed PMID: 10815909.

Kulaksiz H, Eissele R, Rössler D, Schulz S, Höllt V, Cetin Y, Arnold R. Identification of somatostatin receptor subtypes 1, 2A, 3, and 5 in neuroendocrine tumours with subtype specific antibodies. Gut. 2002 Jan;50(1):52-60. PubMed PMID: 11772967; PubMed Central PMCID: PMC1773091.

Lupp A, Hunder A, Petrich A, Nagel F, Doll C, Schulz S. Reassessment of sst(5) somatostatin receptor expression in normal and neoplastic human tissues using the novel rabbit monoclonal antibody UMB-4. Neuroendocrinology. 2011;94(3):255-64. doi: 10.1159/000329876. Epub 2011 Sep27. PubMed PMID: 21952553.

Petrich A, Mann A, Kliewer A, Nagel F, Strigli A, Märtens JC, Pöll F, Schulz S. Phosphorylation of threonine 333 regulates trafficking of the human sst5 somatostatin receptor. Mol Endocrinol. 2013 Apr;27(4):671-82. doi: 10.1210/me.2012-1329. Epub 2013 Feb 15. PubMed PMID: 23418396; PubMed Central PMCID: PMC5416807.

Casar-Borota O, Heck A, Schulz S, Nesland JM, Ramm-Pettersen J, Lekva T, Alafuzoff I, Bollerslev J. Expression of SSTR2a, but not of SSTRs 1, 3, or 5 in somatotroph adenomas assessed by monoclonal antibodies was reduced by octreotide and correlated with the acute and long-term effects of octreotide. J Clin Endocrinol Metab. 2013 Nov;98(11):E1730-9. doi: 10.1210/jc.2013-2145. Epub 2013 Oct 3. PubMed PMID: 24092823.

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