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SST5 (non-phospho), Somatostatin Receptor 5 Antibody

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KO-Validated
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  • 7TM0359N
  • 100 µl
  • Rabbit
The non-phospho-SST5 receptor antibody is directed against the distal end of the... more

The non-phospho-SST5 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human SST5. In can be used to detect total SST5 receptors in Western blots independent of phosphorylation. The non-phospho-SST5 antibody can also be used to isolate and enrich SST5 receptors from tissue and cell lysates. It also detects SST5 in cultured cells and tissue sections by immunohistochemistry. The non-phospho-SST5 antibody has been validated using siRNA knockdown (KO-Validated).

  Alternative Names SST5, SSTR5, Somatostatin Receptor 5 IUPHAR... more

 

Alternative Names SST5, SSTR5, Somatostatin Receptor 5
IUPHAR Target ID  359
UniProt ID  P35346
Western Blot (WB)  1:1000
Immunocytochemistry (ICC) 1:200
Immunohistochemistry (IHC) 1:100
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic peptide with sequence QEATPPAHRRAAANGLMQTSKL corresponds to amino acids 344-364 in human SST3
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Validation of the Somatostatin Receptor 5 in transfected HEK293 cells. Native... more

Figure 1. Validation of the Somatostatin Receptor 5 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Somatostatin Receptor 5 (SST5) were lysed and immunoblotted with the phosphorylation-independent anti-SST5 antibody (7TM0359N-WB) at a dilution of 1:1000.

Figure 2. Western blot analysis of Somatostatin Receptor 5 in neuroendocrine tumors. Human tumor samples (T1, T2) from neuroendocrine tumors were homogenated and immunoblotted using anti-SST5 (non-phospho-Somatostatin-Receptor 5) antibody (7TM0359N-WB) at a dilution of 1:1000.

Figure 3. Immunocytochemical identification of Somatostatin Receptor 5 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Somatostatin Receptor (SST5) were immunocytochemically stained with anti-SST5 (non-phospho-Somatostatin Receptor 5) antibody (7TM0359N-WB) at a dilution of 1:200. Note, SST5 receptors were confined to the plasma membrane in tranfected cells.

Figure 4. Immunohistochemical identification of Somatostatin Receptor 5 in human pancreatic islets. Sections were dewaxed, microwaved in citric acid, and incubated with anti-SST5 (non-phospho-Somatostatin Receptor 5) antibody (7TM0359N-WB) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (ACE), and sections were counterstained with hematoxylin. Note, SST5 receptors were detected at the plasma membrane of nearly all cells in human pancreatic islets.

Figure 5. Immunohistochemical identification of Somastatin Receptor 5 in human anterior pituitary. Sections were dewaxed, microwaved in citric acid, and incubated with anti-SST5 (non-phospho-Somatostatin Receptor 5) antibody (7TM0359N-WB) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (ACE), and sections were counterstained with hematoxylin. Note, SST5 receptors were detected on a distinct population of cells in the anterior pituitary.

Figure 6. Immunohistochemical identification of Somastatin Receptor 5 in human growth-hormone-producing pituitary adenoma tissue. Sections were dewaxed, microwaved in citric acid, and incubated with anti-SST5 (non-phospho-Somatostatin Receptor 5) antibody (7TM0359N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (ACE), and sections were counterstained with hematoxylin. Note, SST5 receptors were uniformly detected at the plasma membrane of nearly all tumor cells.

Figure 7. Immunohistochemical identification of Somastatin Receptor 5 in human pheochromocytoma. Sections were dewaxed, microwaved in citric acid, and incubated with anti-SST5 (non-phospho-Somatostatin Receptor 5) antibody (7TM0359N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (ACE), and sections were counterstained with hematoxylin. Note, SST5 receptors were uniformly detected at the plasma membrane of nearly all cells in pheochromocytoma.

Schulz S, Pauli SU, Schulz S, Händel M, Dietzmann K, Firsching R, Höllt V. Immunohistochemical... more

Schulz S, Pauli SU, Schulz S, Händel M, Dietzmann K, Firsching R, Höllt V. Immunohistochemical determination of five somatostatin receptors in meningioma reveals frequent overexpression of somatostatin receptor subtype sst2A. Clin Cancer Res. 2000 May;6(5):1865-74. PubMed PMID: 10815909.

Kulaksiz H, Eissele R, Rössler D, Schulz S, Höllt V, Cetin Y, Arnold R. Identification of somatostatin receptor subtypes 1, 2A, 3, and 5 in neuroendocrine tumours with subtype specific antibodies. Gut. 2002 Jan;50(1):52-60. PubMed PMID: 11772967; PubMed Central PMCID: PMC1773091.

Lupp A, Hunder A, Petrich A, Nagel F, Doll C, Schulz S. Reassessment of sst(5) somatostatin receptor expression in normal and neoplastic human tissues using the novel rabbit monoclonal antibody UMB-4. Neuroendocrinology. 2011;94(3):255-64. doi: 10.1159/000329876. Epub 2011 Sep27. PubMed PMID: 21952553.

Petrich A, Mann A, Kliewer A, Nagel F, Strigli A, Märtens JC, Pöll F, Schulz S. Phosphorylation of threonine 333 regulates trafficking of the human sst5 somatostatin receptor. Mol Endocrinol. 2013 Apr;27(4):671-82. doi: 10.1210/me.2012-1329. Epub 2013 Feb 15. PubMed PMID: 23418396; PubMed Central PMCID: PMC5416807.

Casar-Borota O, Heck A, Schulz S, Nesland JM, Ramm-Pettersen J, Lekva T, Alafuzoff I, Bollerslev J. Expression of SSTR2a, but not of SSTRs 1, 3, or 5 in somatotroph adenomas assessed by monoclonal antibodies was reduced by octreotide and correlated with the acute and long-term effects of octreotide. J Clin Endocrinol Metab. 2013 Nov;98(11):E1730-9. doi: 10.1210/jc.2013-2145. Epub 2013 Oct 3. PubMed PMID: 24092823.

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