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- Order number: 7TM0279B
- Content: 100 µl
- Host: Rabbit
Serine359/Serine361/Serine363 (S359/S361/S363) is major phosphorylation site of the Sphingosine 1-Phosphate Receptor 5 (S1P5). The pS359/pS361/pS363-S1P5 antibody detects phosphorylation in response to agonists. S359/S361/S363 phosphorylation is likely to be involved in efficient ligand sequestration by S1P5.
Alternative Names | Edg8, endothelial differentiation G protein-coupled receptor 8, NRG-1 |
IUPHAR Target ID | 279 |
UniProt ID | Q9H228 |
Western Blot (WB) | 1:1000 |
Species Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Immunogen | A synthetic phosphopeptide derived from human S1P5 around the phosphorylation site of Ser359/Ser361/Ser363. |
Form | Liquid |
Purification | Antigen affinity chromatography |
Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced Serine359/Serine361/Serine363 phosphorylation of the Sphingosine 1-Phosphate Receptor 5. Upper panel, HEK293 cells stably expressing the S1P5-Receptor (S1P5) were either not exposed or exposed to 1 µM of S1P5-Receptor agonists S1P for 30 minutes. Cells were lysed and immunoblotted with the anti-pS359/pS361/pS363-S1P5 antibody (7TM00279B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-S1P5 antibody to confirm equal loading of the gel.
Be the first to analyze the S1P1 phosphorylation using our anti-pS351/pS353-S1P1 antibody and let us know.