pS366/pS367-S1P5 (phospho-Sphingosine 1-Phosphate Receptor 5 Antibody)

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Agonist-induced Serine366/Serine367 phosphorylation of the Sphingosine 1-Phosphate Receptor 5

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Order number: 7TM0279C
Content: 100 µl
Host: Rabbit

Serine366/Ser367 (S366/S367) is major phosphorylation site of the Sphingosine 1-Phosphate... more

Serine366/Ser367 (S366/S367) is major phosphorylation site of the Sphingosine 1-Phosphate Receptor 5 (S1P5). The pS366/pS367-S1P5 antibody detects phosphorylation in response to agonists. S366/S367 phosphorylation is likely to be involved in efficient ligand sequestration by S1P5.

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Alternative Names Edg8, endothelial differentiation G protein-coupled... more

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Alternative Names	Edg8, endothelial differentiation G protein-coupled receptor 8, NRG-1
IUPHAR Target ID	279
UniProt ID	Q9H228
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human S1P5 around the phosphorylation site of Ser366/Ser367.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine366/Serine367 phosphorylation of the Sphingosine 1-Phosphate... more

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Figure 1. Agonist-induced Serine366/Serine367 phosphorylation of the Sphingosine 1-Phosphate Receptor 5. Upper panel, HEK293 cells stably expressing the S1P5-Receptor (S1P5) were either not exposed or exposed to 1 µM of S1P5-Receptor agonists S1P for 30 minutes. Cells were lysed and immunoblotted with the anti-pS366/pS367-S1P5 antibody (7TM00279C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-S1P5 antibody to confirm equal loading of the gel.

Be the first to analyze the S1P1 phosphorylation using our anti-pS351/pS353-S1P1... more

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Be the first to analyze the S1P1 phosphorylation using our anti-pS351/pS353-S1P1 antibody and let us know.

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