pT377/pT378-V1B (phospho-Vasopressin Receptor 1B Antibody)

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Agonist-induced Threonine377/Threonine378 phosphorylation of the Vasopressin Receptor 1B

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Order number: 7TM0367B
Content: 100 µl
Host: Rabbit

Threonine377/Threonine378 (T377/T378) is major phosphorylation site of the Vasopressin Receptor... more

Threonine377/Threonine378 (T377/T378) is major phosphorylation site of the Vasopressin Receptor 1B (V1B). The pT377/pT378-V1B antibody detects phosphorylation in response to agonists. T377/T378 phosphorylation is likely to be involved in efficient ligand sequestration by V1B.

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Alternative Names AVPR3, V1bR, antidiuretic hormone receptor 1b,... more

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Alternative Names	AVPR3, V1bR, antidiuretic hormone receptor 1b, vasopressin V3 receptor
IUPHAR Target ID	367
UniProt ID	P47901
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human V1B around the phosphorylation site of Thr377/Thr378.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Threonine377/Threonine378 phosphorylation of the Vasopressin Receptor... more

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Figure 1. Agonist-induced Threonine377/Threonine378 phosphorylation of the Vasopressin Receptor 1B. Upper panel, HEK293 cells stably expressing the Vasopressin receptor 1B (V1B) were either not exposed or exposed to 100 nM vasopressin for 30 minutes. Cells were lysed and immunoblotted with the anti-pT377/pT378-V1B antibody (7TM0367B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-V1B antibody to confirm equal loading of the gel.

Be the first to analyze the V2 phosphorylation using our anti-pT359/pT360-V2... more

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Be the first to analyze the V2 phosphorylation using our anti-pT359/pT360-V2 antibody and let us know.

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