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- Order number: 7TM0368C
- Content: 100 µl
- Host: Rabbit
Serine362/Serine363/Serine364 (S362/S363/S364) is major phosphorylation site of the Vasopresssin Receptor 2 (V2). The pS362/pS363/pS364-V2 antibody detects phosphorylation in response to agonists. S362/S363/S364 phosphorylation is likely to be involved in efficient ligand sequestration by V2.
Alternative Names | ADHR, DIR3, renal-type arginine vasopressin receptor |
IUPHAR Target ID | 368 |
UniProt ID | P30518 |
Western Blot (WB) | 1:1000 |
Species Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Immunogen | A synthetic phosphopeptide derived from human V2 around the phosphorylation site of Ser362/Ser363/Ser364. |
Form | Liquid |
Purification | Antigen affinity chromatography |
Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced Serine362/Serine363/Serine364 phosphorylation of the Vasopressin Receptor 2. Upper panel, HEK293 cells stably expressing the Vasopressin receptor 2 (V2) were either not exposed or exposed to 100 nM vasopressin for 30 minutes. Cells were lysed and immunoblotted with the anti-pS362/pS363/pS364-V2 antibody (7TM0368C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-V2 antibody to confirm equal loading of the gel.
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