pS435/pT438-VPAC1 (phospho-VIP Receptor 1 Antibody)

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Agonist-induced and Agonist-independent Serine435/Threonine438 phosphorylation of VAPC1 Receptor

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Order number: 7TM0371C
Content: 100 µl
Host: Rabbit

Serine435/Threonine438 (S435/T438) is a major phosphorylation site of the VPAC1... more

Serine435/Threonine438 (S435/T438) is a major phosphorylation site of the VPAC1 receptor. The pS435/pT438-VPAC1 antibody detects phosphorylation in response to high-efficacy agonists. S422/S425 phosphorylation is a key regulator of VPAC1 desensitization, β-arrestin recruitment and internalization. S435/T438 also undergoes constitutive phosphorylation.

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Alternative Names VPAC1, VIP Receptor 1, Vasoactive Intestinal Peptide... more

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Alternative Names	VPAC1, VIP Receptor 1, Vasoactive Intestinal Peptide Receptor 1
IUPHAR Target ID	371
UniProt ID	P32241
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	-
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human VPAC1 around the phosphorylation site Ser435/Thr438.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced and Agonist-independent Serine435/Threonine438 phosphorylation of... more

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Figure 1. Agonist-induced and Agonist-independent Serine435/Threonine438 phosphorylation of VAPC1 Receptor. Upper panel, HEK293 cells stably expressing the VPAC1-Receptor (VPAC1) were either not exposed or exposed to 1 µM of specific VPAC1-Receptor agonist PACAP-27 for 30 minutes. Cells were lysed and immunoblotted with the anti-pS435/pT438-VPAC1 antibody (7TM0371C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-VPAC1 antibody (7TM0371N) at a dilution of 1:1000 to confirm equal loading of the gel.