β1 (GP-IHC-grade), β1-Adrenoceptor Antibody, Guinea Pig

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Preview: Immunohistochemical identification of ß1-Adrenoceptor in Heart

Preview: Validation of the β1-Adrenoceptor in transfected HEK293 cells

Immunohistochemical identification of ß1-Adrenoceptor in Heart

Validation of the β1-Adrenoceptor in transfected HEK293 cells

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Order number: 7TM0028N-ICGP
Content: 100 µl
Host: Rabbit

The non-phospho- β1 antibody is directed against the carboxyl-terminal tail of human β1 . It... more

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The non-phospho-β1 antibody is directed against the carboxyl-terminal tail of human β1. It can be used to detect total β1 receptors in Western blots independent of phosphorylation. The non-phospho-β1 antibody can also be used to isolate and enrich β1 receptors from cell and tissue lysates.

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Alternative Names β1, B1AR, ADRB1R, β1 Adrenergic... more

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Alternative Names	β1, B1AR, ADRB1R, β1 Adrenergic Receptor, β1-Adrenoceptor
IUPHAR Target ID	28
UniProt ID	P08588
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Guinea Pig / IgG
Class	Polyclonal
Immunogen	A synthetic peptide derived from human β1 with the sequence GPPPSPGAAS.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Immunohistochemical identification of... more

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Figure 1. Immunohistochemical identification of ß1-Adrenoceptor in Heart. Sections were dewaxed, microwaved in citric acid, and incubated with anti-ß1 (ß1-Adrenoceptor) antibody (7TM0028N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.

Figure 2. Validation of the β1-Adrenoceptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the β1-Adrenoceptor (β1) were lysed and immunoblotted with the phosphorylation-independent anti-β1 antibody (7TM0028N-ICGP) at a dilution of 1:1000.