FLAG-Tag, Anti-FLAG Epitope Tag Antibody

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Validation of FLAG-tagged µ-Opioid Receptor in transfected HEK293 cells.

$ 250.00 *

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Delivery time appr. 5-8 days

Order number: 7TM00FLAG
Content: 100 µl
Host: Rabbit

The anti-FLAG tag antibody is directed against the sequence DYKDDDDK. The anti-FLAG antibody can... more

The anti-FLAG tag antibody is directed against the sequence DYKDDDDK. The anti-FLAG antibody can be used to detect total receptors in Western blots independent of phosphorylation. The anti-FLAG tag antibody can also be used to isolate and enrich receptors from cell and tissue lysates. It also detects FLAG-tagged receptors in cultured cells by immunohistochemistry.

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Alternative Names - IUPHAR Target ID - UniProt... more

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Alternative Names	-
IUPHAR Target ID	-
UniProt ID	-
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide with sequence DYKDDDD
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of FLAG-tagged µ-Opioid Receptor in transfected HEK293 cells. Native... more

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Figure 1. Validation of FLAG-tagged µ-Opioid Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the FLAG-tagged µ-Opioid Receptor (FLAG-MOP) were lysed and immunoblotted with the phosphorylation-independent anti-FLAG antibody (7TM00FLAG) at a dilution of 1:1000.

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