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- Order number: 7TM000HA
- Content: 100 µl
- Host: Rabbit
The anti-HA tag antibody is directed against amino acids 98-106 of the human influenza hemagglutinin (HA). The HA tag can used as a general epitope tag in expression vectors. The anti-HA antibody can be used to detect total receptors in Western blots independent of phosphorylation. The anti-HA tag antibody can also be used to isolate and enrich receptors from tissue lysates. It also detects HA-tagged receptors in cultured cells and tissue sections by immunohistochemistry.
| Alternative Names | - |
| IUPHAR Target ID | - |
| UniProt ID | - |
| Western Blot (WB) | 1:1000 |
| Immunocytochemistry (ICC) | 1:200 |
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Immunogen | A synthetic peptide presents amino acids 98-106 of the human influenza hemagglutinin (HA) |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
| Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Validation of HA-tagged µ-Opioid Receptor in stable HEK293 cell lines. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the HA-tagged µ-Opioid Receptor (HA-MOP) were lysed and immunoblotted with the phosphorylation-independent anti-HA antibody (7TM000HA) at a dilution of 1:1000.
Figure 2. Validation of HA-tagged receptors in stable HEK293 cell lines. HEK293 cells stably expressing the HA-tagged Serotonin Receptor 4 (HA-5-HT4), HA-tagged Serotonin Receptor 6 (HA-5-HT6), HA-tagged Serotonin Receptor 2A (HA-5-HT2A), HA-tagged Cholecystokinin Receptor 1 (HA-CCK1) or HA-tagged Cholecystokinin Receptor 2 (HA-CCK2) were lysed and immunoblotted with the phosphorylation-independent anti-HA antibody (7TM000HA) at a dilution of 1:1000.
Figure 3. Validation of HA-tagged receptors in stable HEK293 cell lines. HEK293 cells stably expressing the HA-tagged Adenosin Diphosphate Receptor 4 (HA-P2Y4), HA-tagged Adenosin Diphosphate Receptor 12 (HA-P2Y12), HA-tagged Alpha-1 Adrenergic Receptor A (HA-α1A), HA-tagged Dopamine Receptor 5 (HA-D5) or HA-tagged Histamine H2 receptor (HA-H2) were lysed and immunoblotted with the phosphorylation-independent anti-HA antibody (7TM000HA) at a dilution of 1:1000.
Figure 4. Validation of HA-tagged receptors in stable HEK293 cell lines. HEK293 cells stably expressing the HA-tagged Hypocretin Receptor 1/Orexin Receptor 1 (HA-OX1), HA-tagged Canabinoid Receptor 1 (HA-CB1), HA-tagged Canabinoid Receptor 1 (HA-CB2), HA-tagged Galanin Receptor 1 (HA-GAL1) or HA-tagged G Protein-coupled Estrogen Receptor (HA-GPER) were lysed and immunoblotted with the phosphorylation-independent anti-HA antibody (7TM000HA) at a dilution of 1:1000.
Figure 5. Immuncytochemical verification of HA-tagged receptors in stable HEK293 cell lines. HEK293 cells stably expressing the HA-tagged metabotropic Glutamate Receptor 2 (HA-mGLU2), HA-tagged Calcitonin Receptor-like Receptor (HA-CALCRL), HA-tagged Corticotropin-releasing Factor Receptor 1 (HA-CRF1), HA-tagged orphan G Protein-coupled Receptor 20 (HA-GPR20), metabotropic Glutamate Receptor 4 (HA-mGLU4) or HA-tagged Dopamine receptor 1 (HA-D1) were immunocytochemically stained with the phosphorylation-independent anti-HA antibody (7TM000HA) at a dilution of 1:200. Note, the receptors are located at the plasma membrane of the cells.
Figure 6. Validation of HA-tagged receptors in stable HEK293 cell lines. HEK293 cells stably expressing the HA-tagged C-C chemokine receptor 5 (HA-CCR5), HA-tagged C-C chemokine receptor 7 (HA-CCR7), HA-tagged C-C chemokine receptor 8 (HA-CCR8), HA-tagged C-C chemokine receptor 9 (HA-CCR9), HA-tagged C-C chemokine receptor 10 (HA-CCR10), HA-tagged muscarinic acetylcholine receptor 3 (HA-M3), HA-tagged histamine H1 receptor (HA-H1), HA-tagged C-X3-C motif chemokine receptor 1 (HA-CX3CR1) or HA-tagged hypocretin receptor 1/orexin receptor 1 (HA-OX1) were lysed and immunoblotted with the phosphorylation-independent anti-HA antibody (7TM000HA) at a dilution of 1:1000.
