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PAR2 (IHC-grade), Proteinase-Activated Receptor 2 Antibody

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  • 7TM0348N-IC
  • 100 µl
  • Rabbit
The PAR2 antibody is directed against the distal end of the carboxyl-terminal tail of... more

The PAR2 antibody is directed against the distal end of the carboxyl-terminal tail of mouse, rat and human PAR2. It can be used to detect total PAR2 receptors in Western blots independent of phosphorylation. The PAR2 antibody can also be used to isolate and enrich PAR2 receptors from brain lysates. It also detects PAR2 in cultured cells and tissue sections by immunohistochemistry.

  Alternative Names  coagulation factor II receptor-like 1,... more

 

Alternative Names  coagulation factor II receptor-like 1, Protease-activated receptor-2
IUPHAR Target ID  348
UniProt ID  P55085
Western Blot (WB)  1:1000
Immunohistochemistry (IHC) 1:100
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic peptide corresponding to distal end of carboxy-terminal tail of human PAR2.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Immunohistochemical identification... more

Figure 1. Immunohistochemical identification of Proteinase-Activated Receptor 2 in Kidney. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR32 (GPR32 Receptor) antibody (7TM0348N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.

Figure 2. Validation of the Proteinase-Activated Receptor 2 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Proteinase-Activated Receptor 2 (PAR2) were lysed and immunoblotted with the anti-PAR2 antibody (7TM0348N-IC) at a dilution of 1:1000.

 

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