mSST3 (IHC-grade), Somatostatin Receptor 3 Antibody (Mouse)

Figure 1. Immunohistochemical identification of Somatostatin Receptor 3 in neuronal cilia. Sections were dewaxed, microwaved in citric acid, and incubated with anti-SST3 (Somatostatin Receptor 3) antibody (7TM0357N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, SST3 receptors were uniformly detected at the plasma membrane of nearly all tumor cells.

Figure 2. Immunohistochemical identification of Somatostatin receptor 3 in mouse brain in vivo. Brain sections from SST3-deficient mice (SST3-KO) and their wild-type littermates (SST3-WT) were dewaxed, microwaved in citric acid, and incubated with anti-SST3 (Somatostatin Receptor 3) antibody (7TM0357N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, SST3 receptors were uniformly detected at the plasma membrane of small rod-shaped cells which represent neuronal cilia.

Figure 3. Immunocytochemical identification of Somatostatin Receptor 3 in transfected HEK293 cells. HEK293 cells stably expressing the mouse Somatostatin Receptor (SST3) were either not exposed or exposed to 1 μM SRIF (somatotropin release inhibiting factor) for 30 min and immunocytochemically stained with anti-SST3 (Somatostatin Receptor 3) antibody (7TM0357N-IC) at a dilution of 1:200. Note, SST3 receptors were confined to the plasma membrane in untreated cells (0 min). SST3 receptors were seen in perinuclear clusters of vesicles after 30 min SRIF exposure.

Figure 4. Validation of the Somatostatin Receptor 3 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the mouse Somatostatin Receptor 3 receptor (SST3) were lysed and immunoblotted with the anti-SST3 antibody (7TM0357N-IC) at a dilution of 1:1000.

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