pT306/pT310-FFA2 (IHC grade phospho-FFA2 Antibody)

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Preview: Immunohistochemical Identification of Threonine306/Threonine310 phosphorylation of the FFA Receptor 2 in mouse neuroendocrine cells and peyer's patches.

Preview: Immunocytochemical identifiction of Threonine306/Threonine310 phosphorylation of the FFA Receptor 2

Preview: Agonist-induced Threonine306/Threonine310 phosphorylation of the FFA2 Receptor.

Immunohistochemical Identification of Threonine306/Threonine310 phosphorylation of the FFA Receptor 2 in mouse neuroendocrine cells and peyer's patches.

Immunocytochemical identifiction of Threonine306/Threonine310 phosphorylation of the FFA Receptor 2

Agonist-induced Threonine306/Threonine310 phosphorylation of the FFA2 Receptor.

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Order number: 7TM0226B-IC
Content: 300 µl
Host: Rabbit

Threonine306/Threonine310 (T306/T310) is major phosphorylation site of the FFA 2 Receptor... more

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Threonine306/Threonine310 (T306/T310) is major phosphorylation site of the FFA 2 Receptor (FFA2). The pT306/pT310-FFA2 antibody detects phosphorylation in response to agonists. T306/T310 phosphorylation is likely to be involved in efficient ligand sequestration by FFA2.

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Alternative Names FFAR2, Free Fatty Acid Receptor 2, GPCR3 IUPHAR... more

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Alternative Names	FFAR2, Free Fatty Acid Receptor 2, GPCR3
IUPHAR Target ID	226
UniProt ID	O15552
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	1:200
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human FFA2 around the phosphorylation site of Thr306/Thr310.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Immunohistochemical Identification of Threonine306/Threonine310 phosphorylation of... more

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Figure 1. Immunohistochemical Identification of Threonine306/Threonine310 phosphorylation of the FFA Receptor 2 in mouse neuroendocrine cells and peyer's patches. Sections from transgenic hFFAR2- DREADD- HA- expressing mouse line were treated with 100 µM 4- methoxy- 3- methyl- benzoic acid (MOMBA), fixed, dewaxed, microwaved in citric acid, and incubated with anti-pT306pT310-FFA2 antibody (7TM0226B-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

Figure 2. Immunocytochemical identifiction of Threonine306/Threonine310 phosphorylation of the FFA Receptor 2. Flp-In TREx 293 cells stably expressing hFFA2-HA were either treated with vehicle or 2 mM C3 for 2 min and immunocytochemically stained with the anti-pT306pT310-FFA2 antibody (7TM0226B-IC) at a dilution of 1:200. Note, Threonine306/Threonine310-phosphorylated FFA2 receptors were not detectable in untreated cells (vehicle). Threonine306/Threonine310-phosphorylated FFA2 receptors were seen at the plasma membrane after 2 min C3-treatment.

Figure 3. Agonist-induced Threonine306/Threonine310 phosphorylation of the FFA2 Receptor. Upper panel, parental or Flp-In TREx 293 harbouring human FFA2-eYFP induced to express the receptor construct (+ dox) were treated with vehicle or 2mM propanoic acid (C3) for 5 min. Cells were lysed and receptor construct enriched with a GFP-trap, before immunoblotting with the anti-pT306/pT310-FFA2 antibody (7TM0226B-IC) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with a phosphorylation-independent anti-GFP antibody to confirm equal loading of the gel.

Barki N, Jenkins L, Marsango S, Dedeo D, Bolognini D, Dwomoh L, Abdelmalik AM, Nilsen M,... more

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Barki N, Jenkins L, Marsango S, Dedeo D, Bolognini D, Dwomoh L, Abdelmalik AM, Nilsen M, Stoffels M, Nagel F, Schulz S, Tobin AB, Milligan G. Phosphorylation bar-coding of free fatty acid receptor 2 is generated in a tissue-specific manner. Elife. 2023 Dec 12;12:RP91861. doi: 10.7554/eLife.91861. PMID: 38085667; PMCID: PMC10715726.

Ruhnke N, Beyer AL, Kaemmerer D, Sänger J, Schulz S, Lupp A. Expression of free fatty acid receptor 2 in normal and neoplastic tissues. Exp Mol Pathol. 2024 Jun;137:104902. doi: 10.1016/j.yexmp.2024.104902. Epub 2024 May 23. PMID: 38788249.

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