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pT349/pS350-FFA4 (phospho-FFA4 Antibody)

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Agonist-induced Threonine349/Serine350 phosphorylation of the FFA Receptor 4
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  • 7TM0127B
  • 100 µl
  • Rabbit
Threonine349/Serine350 (T349/S350) is major phosphorylation site of the FFA Receptor 4 (FFA4).... more

Threonine349/Serine350 (T349/S350) is major phosphorylation site of the FFA Receptor 4 (FFA4). The pT349/pS350-FFA4 antibody detects phosphorylation in response to agonists. T349/S350 phosphorylation is likely to be involved in efficient ligand sequestration by FFA4.

  Alternative Names FFAR4, Free Fatty Acid Receptor 4, Omega 3 Fatty Acid... more

 

Alternative Names FFAR4, Free Fatty Acid Receptor 4, Omega 3 Fatty Acid Receptor
IUPHAR Target ID  127
UniProt ID  Q5NUL3
Western Blot (WB)  1:1000
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human FFA4 around the phosphorylation site of Thr349/Ser350.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Threonine349/Serine350 phosphorylation of the FFA Receptor 4. Upper... more

Figure 1. Agonist-induced Threonine349/Serine350 phosphorylation of the FFA Receptor 4. Upper panel, HEK293 cells stably expressing the FFA receptor 4 (FFA4) were either not exposed or exposed to 2 mM TUG-891 for 5 minutes. Cells were lysed and immunoblotted with the anti-pT349/pS350-FFA4 antibody (7TM0127B) at a dilution of 1:1000. Lower panels, blots were stripped and reprobed with a phosphorylation-independent anti-FFA4 antibody to confirm equal loading of the gel.

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