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KOP (non-phospho) κ-Opioid Receptor Antibody

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  • 7TM0318N
  • 100 µl
  • Rabbit
The non-phospho-kappa-opioid receptor antibody is directed against the distal end of the... more

The non-phospho-kappa-opioid receptor antibody is directed against the distal end of the carboxyl-terminal tail of mouse, rat and human KOP. It can be used to detect total KOP receptors in Western blots independent of phosphorylation.

  Alternative Names KOP, OPRK1, κ-Opioid Receptor IUPHAR Target ID... more

 

Alternative Names KOP, OPRK1, κ-Opioid Receptor
IUPHAR Target ID  318
UniProt ID  P41145 (human) P33534 (mouse) P34975 (rat)
Western Blot (WB)  1:1000
Species Reactivity   Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic peptide presents carboxyl-terminal tail of human KOP
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Validation of the κ-Opioid Receptor in transfected HEK293 cells. Native HEK293 cells... more

Figure 1. Validation of the κ-Opioid Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the κ-Opioid Receptor (KOP) were lysed and immunoblotted with the phosphorylation-independent anti-KOP antibody (7TM0318N-WB) at a dilution of 1:1000.

Figure 2. Analysis of dose-dependent κ-Opioid Receptor phosphorylation using two phosphosite-specific antibodies. Upper two panels, HEK293 cells stably expressing the κ-Opioid Receptor (KOP) were either not exposed or exposed to increasing concentrations of selective κ-opioid receptor agonist U50,488H ranging from 0.1 nM to 1 μM for 30 minutes. Cells were lysed and immunoblotted with the anti-pS356/pT357-KOP antibody (7TM0318A) or anti-pT363-KOP antibody (7TM0318B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-KOP antibody (7TM0318N-WB) to confirm equal loading of the gel.

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