![Agonist-induced Serine319/Threonine321 phosphorylation of the IP Prostacyclin Receptor](https://7tmantibodies.com/media/image/25/c3/1a/pS319-pT321-IP-F1_600x600.png)
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- Order number: 7TM0345A
- Content: 100 µl
- Host: Rabbit
Serine319/Threonine321 (S319/T321) is major phosphorylation site of the IP Prostacyclin Receptor (IP). The pS319/pT321-IP antibody detects phosphorylation in response to agonists. S319/T321 phosphorylation is likely to be involved in efficient ligand sequestration by IP.
Alternative Names | Prostanoid IP Receptor, Prostaglandin I2 Receptor, Prostacyclin Receptor |
IUPHAR Target ID | 345 |
UniProt ID | P43119 |
Western Blot (WB) | 1:1000 |
Species Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Immunogen | A synthetic phosphopeptide derived from human IP around the phosphorylation site of Ser319/Thr321. |
Form | Liquid |
Purification | Antigen affinity chromatography |
Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced Serine319/Threonine321 phosphorylation of the IP Prostacyclin Receptor. Upper panel, HEK293 cells stably expressing the IP Prostcyclin receptor (IP) were either not exposed or exposed to 10 µM Treprostinil for 30 minutes. Cells were lysed and immunoblotted with the anti-pS319/pT321-IP antibody (7TM0345A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-IP antibody to confirm equal loading of the gel.
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