![Agonist-induced Threonine353/Threonine356 phosphorylation of NK2 Receptor.](https://7tmantibodies.com/media/image/7e/34/b4/pT353-pT356-NK2-F1_600x600.png)
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- Order number: 7TM0361C
- Content: 100 µl
- Host: Rabbit
Threonine353/Threonine356 (T353/T356) is a major phosphorylation site of the NK2 receptor. The pT353/pT356-NK2 antibody detects phosphorylation in response to high-efficacy agonists. T353/T356 phosphorylation is a key regulator of NK2 desensitization, β-arrestin recruitment and internalization.
Alternative Names | NK2, SKR, TACR2, Neurokinin B Receptor, Substance K Receptor |
IUPHAR Target ID | 361 |
UniProt ID | P21452 |
Western Blot (WB) | 1:1000 |
Immunocytochemistry (ICC) | - |
Species Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Immunogen | A synthetic phosphopeptide derived from human NK2 around the phosphorylation site Thr353/Thr356. |
Form | Liquid |
Purification | Antigen affinity chromatography |
Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced Threonine353/Threonine356 phosphorylation of NK2 Receptor. Upper panel, HEK293 cells stably expressing the NK2-Receptor (NK2) were either not exposed or exposed to 1 µM of specific NK2-Receptor agonist Neurokinin B for 30 minutes. Cells were lysed and immunoblotted with the anti-pT353/pT356-NK2 antibody (7TM0361C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-NK2 antibody to confirm equal loading of the gel.