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pS408/pS409-VPAC2 (phospho-VIP Receptor 2 Antibody)

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  • 7TM0372A
  • 100 µl
  • Rabbit
Serine408/Serine409 (S408/S409) is a major phosphorylation site of the VPAC2 receptor.... more

Serine408/Serine409 (S408/S409) is a major phosphorylation site of the VPAC2 receptor. The pS408/pS409-VPAC2 antibody detects phosphorylation in response to high-efficacy agonists. S408/S409 phosphorylation is a key regulator of VPAC2 desensitization, β-arrestin recruitment and internalization.

  Alternative Names VPAC2, VIP Receptor 2, Vasoactive Intestinal Peptide... more

 

Alternative Names VPAC2, VIP Receptor 2, Vasoactive Intestinal Peptide Receptor 2
IUPHAR Target ID  372
UniProt ID  P41587 (human)
Western Blot (WB)  1:1000
Immunocytochemistry (ICC) 1:200
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from human VPAC2 around the phosphorylation site Ser408/Ser409.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Serine408/Serine409 phosphorylation of VPAC2 Receptor. Upper panel,... more

Figure 1. Agonist-induced Serine408/Serine409 phosphorylation of VPAC2 Receptor. Upper panel, HEK293 cells stably expressing the VPAC2-Receptor (VPAC2) were either not exposed or exposed to 1 µM of specific VPAC-Receptor agonist PACAP-27 for 30 minutes. Cells were lysed and immunoblotted with the anti-pS408/pS409-VPAC2 antibody (7TM0372A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-VPAC2 antibody (7TM0372N) at a dilution of 1:1000 to confirm equal loading of the gel.

Figure 2. Immunocytochemical identification of Serine408/Serine409 phosphorylation of VPAC2-Receptor. HEK293 cells stably expressing the  VPAC2-Receptor (VPAC2) were either not exposed or exposed to 1 µM of specific VPAC-Receptor agonist PACAP-27 and immunocytochemically stained with the anti-pS408/pS409-VPAC2 antibody (7TM0372A) at a dilution of 1:200. Note, Serine408/Serine409-phosphorylated VPAC2 receptors were not detectable in untreated cells (0 min). Serine408/Serine409-phosphorylated VPAC2 receptors were seen at the plasma membrane after 5 min PACAP-27 stimulation.



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