ACKR3 (GP-non-phos, C-Term), Atypical Chemokine Receptor 3 Antibody, Guinea Pig

Figure 1. Validation of the Atypical Chemokine Receptor 3 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Atypical Chemokine Receptor 3 (ACKR3) were lysed and immunoblotted with the phosphorylation-independent c-terminal anti-ACKR3 antibody (7TM0080N-GP) at a dilution of 1:1000. 

Figure 2. Immunohistochemical identification of Atypical Chemokine Receptor 3 in Heart. Sections were dewaxed, microwaved in citric acid, and incubated with anti-ACKR3 (Atypical Chemokine Receptor 3) antibody (7TM0080N-GP) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-guinea pig IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

Figure 3. Immunohistochemical varification of  Atypical Chemokine Receptor 3  antibody in heart. Sections were dewaxed, microwaved in citric acid, and not exposed (left pannel) or exposed to peptide (right pannel) that was used for production of anti-ACKR3 (non-phospho-Atypical Chemokine Receptor 3) antibody (7TM0080N-GP). Sections were then incubated with anti-ACKR3 (non-phospho-Atypical Chemokine Receptor 3) antibody (7TM0080N-GP) at a dilution of 1:100 and sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, only in sections without peptide incubation ACKR3 receptors were uniformly detected at the plasma membrane of cells.