ACKR3 (GP-IHC-grade), Atypical Chemokine Receptor 3 Antibody, Guinea Pig

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Preview: Immunohistochemical identification of Atypical Chemokine Receptor 3 in Heart

Preview: Validation of the Atypical Chemokine Receptor 3 in transfected HEK293 cells

Immunohistochemical identification of Atypical Chemokine Receptor 3 in Heart

Validation of the Atypical Chemokine Receptor 3 in transfected HEK293 cells

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Order number: 7TM0080N-ICGP
Content: 100 µl
Host: Rabbit

The ACKR3 receptor antibody is directed against the distal end of the carboxyl-terminal tail of... more

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The ACKR3 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human Atypical Chemokine Receptor 3. It can be used to detect total ACKR3 receptors in Western blots independent of phosphorylation. The ACKR3 antibody can also be used to detect ACKR3 in formalin-fixed, paraffin-embedded tissue sections by immunohistochemistry.

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Alternative Names ACKR3, CXCR7, Atypical Chemokin Receptor 3, CXC Chemokin... more

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Alternative Names	ACKR3, CXCR7, Atypical Chemokin Receptor 3, CXC Chemokin Receptor 7
IUPHAR Target ID	80
UniProt ID	P25106
Immunohistochemistry (IHC)	1:100
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Guinea Pig / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents distal end of carboxyl-terminal tail of human ACKR3.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Immunohistochemical identification of Atypical Chemokine Receptor 3 in Heart .... more

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Figure 1. Immunohistochemical identification of Atypical Chemokine Receptor 3 in Heart. Sections were dewaxed, microwaved in citric acid, and incubated with anti-ACKR3 (Atypical Chemokine Receptor 3) antibody (7TM0080N-ICGP) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin..

Figure 2. Validation of the Atypical Chemokine Receptor 3 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Atypical Chemokine Receptor 3 (ACKR3) were lysed and immunoblotted with the phosphorylation-independent c-terminal anti-ACKR3 antibody (7TM0080N-ICGP) at a dilution of 1:1000.

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