GCGR (IHC-grade), Glucagon Receptor Antibody

Figure 1. Immunohistochemical identification of the Glucagon Receptor in pancreas. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GCGR (Glucagon Receptor) antibody (7TM0251N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.  

Figure 2. Validation of the Glucagon Receptor  in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Glucagon Receptor  (GCGR) were lysed and immunoblotted with the anti-GCGR antibody (7TM0251N-IC) at a dilution of 1:1000.

Figure 3. Immunohistochemical varification of  Glucagon Receptor antibody in pancreas. Sections were dewaxed, microwaved in citric acid, and not exposed (left pannel) or exposed to peptide (right pannel) that was used for production of anti-GCGR (non-phospho-Glucagon Receptor)) antibody (7TM0251N-IC). Sections were then incubated with anti-GCGR (non-phospho-Glucagon Receptor) antibody (7TM0251N-IC) at a dilution of 1:100 and sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, only in sections without peptide incubation glucagon receptors were uniformly detected at the plasma membrane of cells.