GPR183 (GP-non-phospho), Oxysterol Receptor Antibody, Guinea Pig

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Preview: Indentification of GPR183 Receptor in mouse tissues

Preview: Validation of the GPR183 Receptor in transfected HEK293 cells

Indentification of GPR183 Receptor in mouse tissues

Validation of the GPR183 Receptor in transfected HEK293 cells

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Order number: 7TM0081N-GP
Content: 100 µl
Host: Guinea Pig

The non-phospho-GPR183 receptor antibody is directed against the distal end of the... more

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The non-phospho-GPR183 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human GPR183. It also detects GPR183 in cultured cells and tissue sections by immunohistochemistry. It can be used to detect total GPR183 receptors in Western blots independent of phosphorylation. The GPR183 antibody can also be used to isolate and enrich GPR183 receptors from tissue lysates.

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Alternative Names EBI2, EBV-induced G-protein coupled receptor 2,... more

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Alternative Names	EBI2, EBV-induced G-protein coupled receptor 2, lymphocyte-specific G-protein coupled receptor
IUPHAR Target ID	81
Uniprot ID	P32249
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Guinea Pig / IgG
Class	Polyclonal
Immunogen	Asynthetic peptide corresponding to residues distal end of carboxy-terminal tail of human GPR183.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Indentification of GPR183 Receptor in mouse tissues. Native mouse tissues... more

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Figure 1. Indentification of GPR183 Receptor in mouse tissues. Native mouse tissues were lysed and GPCR receptors were enriched by using WGA-beads (see 7TM Western Blot Protocol). Extracted proteins were immunoblotted with the phosphorylation-independent anti-GPR183 antibody (7TM0081N-GP) at a dilution of 1:1000.

Figure 2. Validation of the GPR183 Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the GPR183 Receptor (GPR183) were not exposed or exposed to 100 nM of specific GPR183-Receptor agonist 7α,25-OHC for 5 minutes, lysed and immunoblotted with the phosphorylation-independent anti-GPR183 antibody (7TM0081N-GP) at a dilution of 1:1000.