GPR183 (IHC-grade), Oxysterol Receptor Antibody

Figure 1. Immunohistochemical identification of GPR183 Receptor in human spleen. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR183 (non-phospho-GPR183 Receptor) antibody (7TM0081N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, GPR183 receptors were uniformly detected at the plasma membrane of nearly all cells.

Figure 2. Immunohistochemical varification of GPR183 Receptor antibody in human spleen. Sections were dewaxed, microwaved in citric acid, and not exposed (left pannel) or exposed to peptide (right pannel) that was used for production of anti-GPR183 (non-phospho-GPR183 Receptor) antibody (7TM0081N-IC). Sections were then incubated with anti-GPR183 (non-phospho-GPR183 Receptor) antibody (7TM0081N-IC) at a dilution of 1:100 and sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, only in sections without peptide incubation GPR183 receptors were uniformly detected at the plasma membrane of nearly all cells.

Figure 3. Validation of the GPR183 Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the GPR183 Receptor (GPR183) were not exposed or exposed to 100 nM of specific GPR183-Receptor agonist 7α,25-OHC for 5 minutes, lysed and immunoblotted with the phosphorylation-independent anti-GPR183 antibody (7TM0081N-IC) at a dilution of 1:1000.