GPR15 (IHC-grade), G Protein-Coupled Receptor 15 Antibody

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Preview: Immunohistochemical identification of GPR15 Receptor in kidney

Preview: Immunohistochemical identification of GPR15 Receptor in Pancreas

Preview: Validation of the GPR15 Receptor in transfected HEK293 cells

Immunohistochemical identification of GPR15 Receptor in kidney

Immunohistochemical identification of GPR15 Receptor in Pancreas

Validation of the GPR15 Receptor in transfected HEK293 cells

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Order number: 7TM0087N-IC
Content: 100 µl
Host: Rabbit

The GPR15 antibody is directed against the distal end of the carboxyl-terminal tail of mouse,... more

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The GPR15 antibody is directed against the distal end of the carboxyl-terminal tail of mouse, rat and human GRP15. It can be used to detect total GPR15 receptors in Western blots independent of phosphorylation. The GPR15 antibody can also be used to isolate and enrich GPR15 receptors from brain lysates. It also detects GPR15 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names BOB, Brother of Bonzo IUPHAR Target ID ... more

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Alternative Names	BOB, Brother of Bonzo
IUPHAR Target ID	87
UniProt ID	P49685
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide corresponding to distal end of carboxy-terminal tail of human GPR15.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Immunohistochemical identification of GPR15 Receptor in kidney. ... more

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Figure 1. Immunohistochemical identification of GPR15 Receptor in kidney. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR15 (GPR15 Receptor) antibody (7TM0087N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.

Figure 2. Immunohistochemical identification of GPR15 Receptor in Pancreas. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR15 (GPR15 Receptor) antibody (7TM0087N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.

Figure 3. Validation of the GPR15 Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the GPR15 Receptor (GPR15) were lysed and immunoblotted with the phosphorylation-independent anti-GPR15 antibody (7TM0087N-IC) at a dilution of 1:1000.

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