GPR27 (IHC-grade), G Protein-Coupled Receptor 27 Antibody

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Preview: Immunohistochemical identification of GPR27 Receptor in small intestine

Preview: Validation of the GPR27 Receptor in transfected HEK293 cells

Immunohistochemical identification of GPR27 Receptor in small intestine

Validation of the GPR27 Receptor in transfected HEK293 cells

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Order number: 7TM0097N-IC
Content: 100 µl
Host: Rabbit

The GPR27 antibody is directed against the distal end of the carboxyl-terminal tail of mouse,... more

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The GPR27 antibody is directed against the distal end of the carboxyl-terminal tail of mouse, rat and human GRP27. It can be used to detect total GPR27 receptors in Western blots independent of phosphorylation. The GPR27 antibody can also be used to isolate and enrich GPR27 receptors from brain lysates. It also detects GPR27 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names SREB1 IUPHAR Target ID 97... more

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Alternative Names	SREB1
IUPHAR Target ID	97
UniProt ID	Q9NS26
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide corresponding to distal end of carboxy-terminal tail of human GPR27.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Immunohistochemical identification of GPR27 Receptor in small... more

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Figure 1. Immunohistochemical identification of GPR27 Receptor in small intestine. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR27 (GPR27 Receptor) antibody (7TM0097N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.

Figure 2. Validation of the GPR27 Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the GPR27 Receptor (GPR27) were lysed and immunoblotted with the phosphorylation-independent anti-GPR27 antibody (7TM0097N-IC) at a dilution of 1:1000.