S1P1 (IHC-grade), Sphingosine 1-Phosphate Receptor 1 Antibody

Figure 1. Immunohistochemical identification of S1P1 Receptor in human spleen. Sections were dewaxed, microwaved in citric acid, and incubated with anti-S1P1 (non-phospho-Sphingosine 1-Phosphate Receptor 1) antibody (7TM0275N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

Figure 2. Immunohistochemical identification of S1P1 Receptor in human kidney. Sections were dewaxed, microwaved in citric acid, and incubated with anti-S1P1 (non-phospho-Sphingosine 1-Phosphate Receptor 1) antibody (7TM0275N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

Figure 3. Immunohistochemical varification of S1P1 Receptor antibody in human spleen. Sections were dewaxed, microwaved in citric acid, and not exposed (left pannel) or exposed to peptide (right pannel) that was used for production of anti-S1P1 (non-phospho-S1P1 Receptor) antibody (7TM0275N-IC). Sections were then incubated with anti-S1P1 (non-phospho-S1P1 Receptor) antibody (7TM0275N-IC) at a dilution of 1:100 and sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, only in sections without peptide incubation S1P1 receptors were uniformly detected at the plasma membrane of nearly all cells.

Figure 4. Immunohistochemical varification of S1P1 Receptor antibody in human kidney. Sections were dewaxed, microwaved in citric acid, and not exposed (left pannel) or exposed to peptide (right pannel) that was used for production of anti-S1P1 (non-phospho-S1P1 Receptor) antibody (7TM0275N-IC). Sections were then incubated with anti-S1P1 (non-phospho-S1P1 Receptor) antibody (7TM0275N-IC) at a dilution of 1:100 and sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, only in sections without peptide incubation S1P1 receptors were uniformly detected at the plasma membrane of nearly all cells.

Figure 5. Validation of the S1P1 Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the S1P1 Receptor (S1P1) were lysed and immunoblotted with the phosphorylation-independent anti-S1P1 antibody (7TM0275N-IC) at a dilution of 1:100.