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- Order number: 7TM0124N
- Content: 100 µl
- Host: Rabbit
The non-phospho-LPA5 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human LPA5. It also detects LPA5 in cultured cells and tissue sections by immunohistochemistry. It can be used to detect total LPA5 receptors in Western blots independent of phosphorylation. The LPA5 antibody can also be used to isolate and enrich LPA5 receptors from tissue lysates.
Alternative Names | Edg7, endothelial differentiation gene 7, lysophosphatidic acid G-protein-coupled receptor 7 |
IUPHAR Target ID | 274 |
Uniprot ID | Q9UBY5 |
Western Blot (WB) | 1:1000 |
Species Reactivity | Human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Immunogen | A synthetic peptide corresponding to c-terminal end of human LPA3. |
Form | Liquid |
Purification | Antigen affinity chromatography |
Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Validation of the Lysophosphatidic Acid Receptor 3 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the LPA3 Receptor (LPA3) were lysed and immunoblotted with the phosphorylation-independent anti-LPA3 antibody (7TM0274N) at a dilution of 1:100.