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- Order number: 7TM0317D
- Content: 100 µl
- Host: Rabbit
Threonine361/Serine363 (T361/S363) is the primary phosphorylation site in a hierarchical phosphorylation cascade of the delta-opioid receptor (DOP). The pT361/pS363-DOP antibody detects phosphorylation in response to high- and low-efficacy agonists but not after PKC activation. T361/S363 phosphorylation is a key regulator of DOP desensitization, β-arrestin recruitment and internalization.
Alternative Names | DOP, OPRD1, δ-Opioid Receptor |
IUPHAR Target ID | 317 |
UniProt ID | P41143 (human) P32300 (mouse) P33533 (rat) |
Western Blot (WB) | 1:1000 |
Species Reactivity | Human, Mouse, Rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Immunogen | A synthetic phosphopeptide derived from human DOP around the phosphorylation site of Thr361/Ser363. |
Form | Liquid |
Purification | Antigen affinity chromatography |
Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced Threonine361/Serine363 phosphorylation of the δ-Opioid receptor. Upper panel, HEK293 cells stably expressing the δ-Opioid Receptor (DOP) were either not exposed or exposed to 10 μM DPDPE ([D-Pen2,D-Pen5]-enkephalin) for 30 minutes. Cells were lysed and immunoblotted with the anti-pT361/pS363-DOP antibody (7TM0317D) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-DOP antibody (7TM0317N) at a dilution of 1:1000 to confirm equal loading of the gel.